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normal human fibroblast cells crl1554  (ATCC)


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    ATCC normal human fibroblast cells crl1554
    Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells <t>(CRL1554)</t> (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.
    Normal Human Fibroblast Cells Crl1554, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+fibroblast+cells+crl1554/BUD-8/pmc04464250-72-16-24
    Average 93 stars, based on 51 article reviews
    normal human fibroblast cells crl1554 - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer"

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer

    Journal: Cancer Cell International

    doi: 10.1186/s12935-015-0194-0

    Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells (CRL1554) (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.
    Figure Legend Snippet: Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells (CRL1554) (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.

    Techniques Used: Inhibition, MTT Assay, Incubation, Staining, Control

    Related Articles

    Inhibition:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer
    Article Snippet: Human colorectal cancer cell lines (SW1116 and SW837), human breast cancer cell lines (HTB26, HTB132), and normal human fibroblast cells (CRL1554) were obtained from American Type Culture Collection (ATCC; VA, USA).. SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.

    MTT Assay:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer
    Article Snippet: Human colorectal cancer cell lines (SW1116 and SW837), human breast cancer cell lines (HTB26, HTB132), and normal human fibroblast cells (CRL1554) were obtained from American Type Culture Collection (ATCC; VA, USA).. SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.

    Incubation:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer
    Article Snippet: Human colorectal cancer cell lines (SW1116 and SW837), human breast cancer cell lines (HTB26, HTB132), and normal human fibroblast cells (CRL1554) were obtained from American Type Culture Collection (ATCC; VA, USA).. SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.

    Staining:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer
    Article Snippet: Human colorectal cancer cell lines (SW1116 and SW837), human breast cancer cell lines (HTB26, HTB132), and normal human fibroblast cells (CRL1554) were obtained from American Type Culture Collection (ATCC; VA, USA).. SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.

    Control:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer
    Article Snippet: Human colorectal cancer cell lines (SW1116 and SW837), human breast cancer cell lines (HTB26, HTB132), and normal human fibroblast cells (CRL1554) were obtained from American Type Culture Collection (ATCC; VA, USA).. SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.SW1116, SW837, HTB26 and HTB132 cells were cultured in 90% Leibovitz’s L15 medium supplemented with 10% heat-inactivated fetal bovine serum and grown at 37°C in a non-CO 2 incubator.



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    normal human fibroblast cells crl1554  (ATCC)
    93
    ATCC normal human fibroblast cells crl1554
    Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells <t>(CRL1554)</t> (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.
    Normal Human Fibroblast Cells Crl1554, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+fibroblast+cells+crl1554/BUD-8/pmc04464250-72-16-24
    Average 93 stars, based on 1 article reviews
    normal human fibroblast cells crl1554 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells (CRL1554) (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.

    Journal: Cancer Cell International

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris , on human breast and colorectal cancer

    doi: 10.1186/s12935-015-0194-0

    Figure Lengend Snippet: Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells (CRL1554) (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.

    Article Snippet: Human colorectal cancer cell lines (SW1116 and SW837), human breast cancer cell lines (HTB26, HTB132), and normal human fibroblast cells (CRL1554) were obtained from American Type Culture Collection (ATCC; VA, USA).

    Techniques: Inhibition, MTT Assay, Incubation, Staining, Control